منابع مشابه
Incorporation of Lysozyme into Liposomes
To define a model for enzyme latency in vitro, the capture was studied of egg white lysozyme by liposomes (phospholipid spherules in the smectic mesophase). Gel filtration resolved liposomes formed in the absence of enzyme from free enzyme when both were chromatographed immediately after mixing. In contrast, if liposomes were swollen in the presence of lysozyme, subsequent chromatography disclo...
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Innovative mobile-based tools have made new opportunities for learners to spend more quality time inside the classrooms. Keeping this in mind, the present study sought to examine the effect of the flipped classroom on developing EFL learners’ speaking and listening skills. To do so, 60 EFL students from two universities in Iran were assigned into one of the classes of flipped and conventional g...
متن کاملRole of charge and fluidity in the incorporation of cytochrome oxidase into liposomes.
Inactive, lipid-depleted cytochrome oxidase was rapidly activated upon incubation with either neutral, basic, or acidic liposomes. When incubated with 0.5 to 10 times its weight in liposomes, the enzyme bound 20% of its weight in lipids. This amount of bound lipids probably corresponds to the “annular lipids” postulated to be tightly bound to cytochrome oxidase (Jest, P. C., Griffith, 0. H., Ca...
متن کاملIncorporation of mannose 6-phosphate receptors into liposomes. Receptor topography and binding of alpha-mannosidase.
A receptor that binds the lysosomal enzyme alpha-mannosidase via mannose 6-phosphate moieties (mannose 6-phosphate receptor) was purified from Swarm-rat chondrosarcoma and bovine liver microsomal membranes. Receptor-reconstituted liposomes were prepared by dialysis of taurodeoxycholate-dispersed lipids with purified mannose 6-phosphate receptor. Liposomes appeared by electron microscopy as 60-1...
متن کاملIncorporation of recombinant gamma interferon into liposomes enhances its ability to induce peritoneal macrophage antitoxoplasma activity.
In this study we compared the ability of free- and liposome-incorporated murine recombinant gamma interferon (rIFN-gamma) to enhance peritoneal macrophage H2O2 release and antitoxoplasma activity in vitro. rIFN-gamma was efficiently (37 to 47%) incorporated into multilamellar vesicles composed of phosphatidylglycerol/cholesterol in a 2:1 molar ratio. The amount of rIFN-gamma incorporated into m...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62994-1